Description
Lightning Blocking Buffer
Method :
1. Soaking the transferred PVDF-membrane in the blocking buffer for 10-30sec at RT under agitation. ( 1~3 mins for NC membrane )
2. Wash the membrane in PBS-T or TBS-T buffer for 2 x 3mins at RT under agitation.
Lightning Stripping Buffer
Method :
1.Membrane is incubated and shook for 1-3 mins in a vessel with Stripping Buffer for removing the antibodies.
2.After the incubation with Stripping Buffer the membrane has to be washed with Washing Buffer and can be used for repeated detection.