Description
The Total RNA Extraction Kit is specially designed for purification of total RNA from bacterial, cultured cells and fresh human whole blood. The method uses detergents and a chaotropic salt to lyse the cells and inactivate RNase. Lysate is clarified with provided lysate filter columns. RNA in chaotropic salt solutions binds to the glass fiber matrix of the RB columns. Following washing off of contaminants, the purified RNA is eluted by RNase-free water. ssRNA and dsRNA of > 200 bps to 1000's of bps in length are efficiently purified. Purified RNA is ready for RT-PCR, northern blotting, primer extension and cDNA library construction.